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small interfering rna (sirna) plasmids directed against mccc1 and mavs  (Ribobio co)
90
Ribobio co small interfering rna (sirna) plasmids directed against mccc1 and mavs
( A ) A549 cells were transfected <t>with</t> <t>siRNA</t> targeting <t>MAVS</t> or nonsense control siRNA for 48 h, and MAVS mRNA and protein levels were measured by qPCR and western blotting, respectively. *p < 0.05 (one-way ANOVA). ( B ) A549 cells were co-transfected with vector alone or MCCC1 expression vector and the indicated siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( C ) A549 cells were co-transfected with control vector alone or MAVS expression plasmid and MCCC1-specific siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, the cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( D – H ) HEK293T cells were co-transfected with HA-MCCC1 and FLAG-MAVS expression vectors ( D ), HA-TRAF6 and FLAG-MCCC1 expression vectors ( E ), HA-MCCC1 and FLAG-TRADD expression vectors ( F ), HA-MCCC1 and FLAG-TRAF3 expression vectors ( G ), or HA-MCCC1 and FLAG-NEMO expression vectors ( H ) for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. All experiments were repeated at least three times with consistent results.
Small Interfering Rna (Sirna) Plasmids Directed Against Mccc1 And Mavs, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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small interfering rna (sirna) plasmids directed against mccc1 and mavs - by Bioz Stars, 2026-11
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( A ) A549 cells were transfected with siRNA targeting MAVS or nonsense control siRNA for 48 h, and MAVS mRNA and protein levels were measured by qPCR and western blotting, respectively. *p < 0.05 (one-way ANOVA). ( B ) A549 cells were co-transfected with vector alone or MCCC1 expression vector and the indicated siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( C ) A549 cells were co-transfected with control vector alone or MAVS expression plasmid and MCCC1-specific siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, the cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( D – H ) HEK293T cells were co-transfected with HA-MCCC1 and FLAG-MAVS expression vectors ( D ), HA-TRAF6 and FLAG-MCCC1 expression vectors ( E ), HA-MCCC1 and FLAG-TRADD expression vectors ( F ), HA-MCCC1 and FLAG-TRAF3 expression vectors ( G ), or HA-MCCC1 and FLAG-NEMO expression vectors ( H ) for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. All experiments were repeated at least three times with consistent results.

Journal: Scientific Reports

Article Title: Methylcrotonoyl-CoA carboxylase 1 potentiates RLR-induced NF-κB signaling by targeting MAVS complex

doi: 10.1038/srep33557

Figure Lengend Snippet: ( A ) A549 cells were transfected with siRNA targeting MAVS or nonsense control siRNA for 48 h, and MAVS mRNA and protein levels were measured by qPCR and western blotting, respectively. *p < 0.05 (one-way ANOVA). ( B ) A549 cells were co-transfected with vector alone or MCCC1 expression vector and the indicated siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( C ) A549 cells were co-transfected with control vector alone or MAVS expression plasmid and MCCC1-specific siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids and pRL-TK (internal control). Twenty-four hours after transfection, the cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01, no sign., no significant difference. (one-way ANOVA). ( D – H ) HEK293T cells were co-transfected with HA-MCCC1 and FLAG-MAVS expression vectors ( D ), HA-TRAF6 and FLAG-MCCC1 expression vectors ( E ), HA-MCCC1 and FLAG-TRADD expression vectors ( F ), HA-MCCC1 and FLAG-TRAF3 expression vectors ( G ), or HA-MCCC1 and FLAG-NEMO expression vectors ( H ) for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. All experiments were repeated at least three times with consistent results.

Article Snippet: The small interfering RNA (siRNA) plasmids directed against MCCC1 and MAVS were purchased from Guangzhou RiboBio Group (Guangzhou, China).

Techniques: Transfection, Control, Western Blot, Plasmid Preparation, Expressing, Luciferase, Infection, Immunoprecipitation

( A , B ) Western analysis of results of co-immunoprecipitation of MCCC1 and MAVS ( A ) and MCCC1 and TRAF6 ( B ) from SeV-infected or uninfected A549 cells using anti-MCCC1 ( A ) and anti-TRAF6 ( B ) antibody or control IgG. ( C , D ) Confocal immunofluorescence microscopic analysis of MCCC1 and MAVS in A549 cells endogenous ( C ) and 24 h after transfection with DsRed-MCCC1 and GFP-MAVS expression plasmids ( D ). ( E ) Confocal immunofluorescence microscopic analysis of MCCC1 and TRAF6 in A549 cells 24 h after transfection with HA-TRAF6 expression vector. Nuclei were counterstained with DAPI. Scale bar, 10 μm. All experiments were repeated at least three times with consistent results.

Journal: Scientific Reports

Article Title: Methylcrotonoyl-CoA carboxylase 1 potentiates RLR-induced NF-κB signaling by targeting MAVS complex

doi: 10.1038/srep33557

Figure Lengend Snippet: ( A , B ) Western analysis of results of co-immunoprecipitation of MCCC1 and MAVS ( A ) and MCCC1 and TRAF6 ( B ) from SeV-infected or uninfected A549 cells using anti-MCCC1 ( A ) and anti-TRAF6 ( B ) antibody or control IgG. ( C , D ) Confocal immunofluorescence microscopic analysis of MCCC1 and MAVS in A549 cells endogenous ( C ) and 24 h after transfection with DsRed-MCCC1 and GFP-MAVS expression plasmids ( D ). ( E ) Confocal immunofluorescence microscopic analysis of MCCC1 and TRAF6 in A549 cells 24 h after transfection with HA-TRAF6 expression vector. Nuclei were counterstained with DAPI. Scale bar, 10 μm. All experiments were repeated at least three times with consistent results.

Article Snippet: The small interfering RNA (siRNA) plasmids directed against MCCC1 and MAVS were purchased from Guangzhou RiboBio Group (Guangzhou, China).

Techniques: Western Blot, Immunoprecipitation, Infection, Control, Immunofluorescence, Transfection, Expressing, Plasmid Preparation